Microbio Practical Exam~~~Finally!!!
October 5th, 2006 by jasonlawThursday, 05/10/2006
The first thing I realised when I woke up this morning was palpitations. Why? Cause I’ll be having Microbio Lab Exam at 10a.m. later today and there’s still loads I’ve yet to cover. Worst still, I’ve just found out last night that Mario came in to class to give some tips for this practical, which I missed. I didn’t know that we had to memorise the composition of each medium as well…
I headed to the library the moment I reached IMU. There’s this particular Practical Microbiology book that I wanted to read before siting for the exam later. Went to the Red Spot section only to find that someone had already borrowed it (Damn!!!). There wasn’t any other book that was useful, so I thought there was no point to stay there. At the entrance, I bumped into Vinegar and found out that he was the fella who had borrowed it. Hmmm…Can’t say I’m surprised…
So, read for a while till about 9a.m. then headed up to the 4th floor to discuss further with the gang. Had quite a few laughs regarding Cetrimide, Chocolate Agar, MgCl, Potassium Sulphate, Belgium Chocolates (You guys who were there, faham-faham lar). Headed to MDL 7 at about 9.50 a.m. to find out that I was supposed to be in MDL 5.
I sat opposite Laura and was given Nutrient Agar with some white dots growing in it. For some reason, I thought that Nutrient Agar was just the general name for the medium and it didn’t cross my mind that Nutrient Agar IS the actual medium for bacterial growth. I panicked, trying to rack my brains to recall what kind of bacterial would give white circular colonies BUT nothing came to my mind. Out of desperation, I took off the cover lid and took a sniff BUT i inhaled TOO HARD. It was a really stupid thing to do which I regretted immediately since loads of bacterial spores entered through my nostril into my airways!!!
I coughed and spluttered since it was really irritating and was worried that they might actually stick to my airways. Dunno whether I imagined it or what, but I suddenly had nose block and my eyes started to stream. Anyway, I stared at my petri dish for a while and decided that it was useless. I just didn’t know how it was possible to guess the organism without first staining it. So I performed Gram staining. When I put the processed slide under the microscope, I could see the colonies under 4X. And 10X. BUT when I turned it to 40X, the colonies went missing!!! So I thought maybe I’ll try my luck at 100X just to find them GONE as well…So I worked backwords, viewed under 40X (no result), under 10X (no result, somehow) and I was like "Oh, shit!!!" view it under 4X, still missing…
I glanced up at Laura to see that she also faced the same problem and started to redo her Gram stain. So I repeated Gram staining and left the safranin solution a little longer this time. Luckily I could find the damn colonies this time and proceeded to answer Question 2. But there was still Question 1 which I didn’t know how to answer. Thanks to Laura, she whispered to me the answer. Then Wai Leong also came over to "discuss" with us without Mario finding out. Why? He was so engrossed in chit-chatting with the new lecturer to notice us…Muahahahahaha…Anyway, after the lab exam, I started sniffing and my eyes smarted once in a while but was so glad that it was finally over!!!
Today when I was travelling back by LRT, I saw this LaLa Mui. She LOOKED SO HORRIBLE!!! She had a "coconut cut" for her fringe with ugly frizzly hair. She carried a HUMONGOUS bag with black and white spots like a New Zealand COW, wore torn jeans with ripped ends at her knees. If you think that was bad enough, she wore black stockings that stretches up to her knees inside white shoes with sparkling purple shoe laces!!! And she has the THICKEST eye shadows I’ve ever seen, giving the impression that she hasn’t been sleeping for the past few weeks…Really…some people just have no DRESS SENSE…Haiyoh…









